Journal: Frontiers in Ophthalmology

Article Title: Interleukin-1β activates matrix metalloproteinase-2 to alter lacrimal gland myoepithelial cell structure and function

doi: 10.3389/fopht.2024.1415002

Figure Lengend Snippet: Role of c-Jun N-terminal kinase (JNK) in the interleukin-1b (IL-1b)-induced increase of matrix metalloproteinase-2 (MMP-2) protein expression. Lysates were prepared from control myoepithelial cells (MECs) and from MECs treated with IL-1β- and IL-1β plus the JNK inhibitor SP600125. Samples were subjected to SDS-PAGE followed by Western blotting using a monoclonal anti-MMP-2 antibody or a polyclonal anti-β-actin antibody, used as a loading control. (A, C) Representative blots. (B, D) Data from four to six independent experiments are presented as the mean ± SD.

Article Snippet: The rabbit monoclonal antibody against β-actin (clone HL1926, 1:10,000 dilution) was from GeneTex (Irvine, CA, USA).

Techniques: Expressing, Control, SDS Page, Western Blot

Journal: Environmental Health and Preventive Medicine

Article Title: Sasa veitchii extract protects against carbon tetrachloride-induced hepatic fibrosis in mice

doi: 10.1186/s12199-018-0739-7

Figure Lengend Snippet: Effect of SE on levels of hepatic αSMA levels. Animals treated as described in Fig. were euthanized at 72 h post intraperitoneal injection, and liver tissues were isolated. Proteins were isolated, and western blotting was performed. β-actin was used as the internal control. Band intensity was measured using ImageJ software.

Article Snippet: Next, the membrane was incubated with mouse anti-α-smooth muscle actin (anti-αSMA) monoclonal antibody (Santa Cruz), mouse anti-β-actin monoclonal antibody (MBL, Aichi, Japan), rabbit anti-c-Jun N-terminal kinase (JNK) polyclonal antibody, rabbit anti-phospho-JNK monoclonal antibody, rabbit-anti extracellular signal-regulated kinase (ERK) 1/2 monoclonal antibody, rabbit anti-phospho-ERK1/2 monoclonal antibody, rabbit-anti p38 monoclonal antibody, rabbit anti-phospho-p38 monoclonal antibody, peroxidase-conjugated anti-rabbit IgG (Cell Signaling Technology, Beverly, MA, USA), and peroxidase-conjugated anti-mouse IgG (BioRad) using previously described conditions [ ].

Techniques: Injection, Isolation, Western Blot, Software

Journal: PLoS ONE

Article Title: A Novel Zebrafish Model to Provide Mechanistic Insights into the Inflammatory Events in Carrageenan-Induced Abdominal Edema

doi: 10.1371/journal.pone.0104414

Figure Lengend Snippet: (A) Western blots for MPO, TNF-α, iNOS, and β-actin proteins from adult zebrafish; (B) relative density of the MPO immunoblot; (C) relative density of the TNF-α immunoblot; (D) relative density of the iNOS immunoblot. There are 3 groups: vehicle+vehicle: i.p. injection first vehicle (20 µL PBS) 1 h before i.p. second vehicle (20 µL PBS); vehicle+carra: i.p. vehicle (20 µL PBS) 1 h before i.p. 1.5% carrageenan (20 µL); MP+carra: i.p. 1 µg MP (20 µL) 1 h before i.p. 1.5% carrageenan (20 µL). The abdominal samples from adult zebrafish were collected at 24 h after the second injection (as well as 25 h after the first injection). Relative intensity of the i.p. vehicle plus i.p. 1.5% carrageenan group was taken to be 100%. Band intensities of MPO, TNF-α, and iNOS were quantified by densitometry and are indicated as a percentage change relative to that of the vehicle plus carrageenan group. Western blotting of the abdominal tissue homogenate revealed that injection of carrageenan evoked significant upregulation of MPO, TNF-α, and iNOS proteins at 24 h compared with that of the vehicle plus vehicle group. MP administered at a dose of 1 µg (i.p.) 1 h prior to i.p. 1.5% carrageenan significantly reduced carrageenan-induced upregulation of MPO, TNF-α, and iNOS. Western blotting of β-actin was performed to verify that equivalent amounts of protein were loaded in each lane. These experiments were repeated 3 times. Ca: carrageenan; MP: methylprednisolone; MPO: myeloperoxidase, TNF-α: tumor necrosis factor-α; iNOS: inducible nitric oxide synthase. * P <0.05 compared with the i.p. vehicle plus i.p. vehicle group; # P <0.05 compared with the i.p. vehicle plus i.p. 1.5% carrageenan group.

Article Snippet: At the end, the PVDF membranes were reprobed with an anti-β-actin antibody (1∶2500 dilution; GeneTex, San Antonio, TX, USA; catalog no. GTX124500; polyclonal rabbit antibody).

Techniques: Western Blot, Injection

Journal: Oncotarget

Article Title: A set of cancer stem cell homing peptides associating with the glycan moieties of glycosphingolipids

doi: 10.18632/oncotarget.24960

Figure Lengend Snippet: (A) The relative intensities of glycan 66 (Gal-β-1,3-GalNAc-β-), glycan 67 (Gal-β-1,3-(Neu5Ac-α-2,6)-GalNAc-β-), glycan 68 (Neu5Ac-α-2,6-Gal-β-1,3-GalNAc-β-), glycan 69 (Neu5Ac-α-2,6-Gal-β-1,3-(Neu5Ac-α-2,6)-GalNAc-β-), and glycan 70 (Neu5Ac-α-2,3-Gal-β-1,3-(Neu5Ac-α-2,6)-GalNAc-β-) signals collected from CSC HP-10, CSC HP-11, and CSC HP-hP2 chips were plotted. (B) Western blotting analysis of ST6GalNAc5 and ST6Gal1 protein expression in PANC-1 CCs and CSCs. β-actin was illustrated as internal control.

Article Snippet: The primary antibodies, rabbit polyclonal antibodies against ST6GalNAc5 (GTX45949), ST6Gal1 (GTX104018) and β-actin (GTX109369), and secondary HRP-conjugated goat anti-rabbit antibody were purchased from GeneTex.

Techniques: Glycoproteomics, Western Blot, Expressing, Control